Total Internal Reflectance Fluorescence Microscope

Location: PAIS 2409

Equipment List

Microscope body

• Olympus IX71 Microscope Base.

• MadCityLabs Nano-LPS100, XYZ piezo stage, travel 100 $\mathrm{\mu m}$.

Objective

• Olympus APO N 60X oil Immersion, NA 1.49, WD 0.1 mm, FN 22.

Cameras

• Hamamatsu C11440-22CU, sCMOS camera, 2048x2048 pixels, pixel size 6.5 $\mathrm{\mu m}$.

Illumination

• Crystal Laser, DL-405-010-O, 405 nm laser, 10 mW.

• Thorlabs, HL6366DG, 642 nm laser, 80 mW.

• Newport Spectra Physics, PC15108, 488 nm laser, 100 mW.

• Coherent Sapphire, 561-100 CW CDRH, 561 nm laser, 100 mW.

Other equipments

• National Instrument, PCIe-6323, DAQ card.

Filters

• Semrock, Di01-R405/488/561/635-25x36, quad-band dichroic mirror

• Semrock, FF01-446/523/600/677-25, quad-band band pass filter

• Semrock, FF01-685/40-25, band pass filter

• Semrock, FF01-525/30-25, band pass filter