Single Molecule Localiation Micrsocopy (SMLM) Super-resolution Micrsocope

Location: PAIS 2409

Equipment List

Microscope body

• Home built base.

• Thorlabs, MAX381/M, 3-Axis NanoMax stage, travel 4 mm with stepper motor and 20 $\mathrm{\mu m}$ with piezo.

Objective

• Olympus, UPLSAPO 100XS, silicone oil Immersion, NA 1.35, WD 0.2 mm, FN 22.

Cameras

• Hamamatsu, C11440-22CU, sCMOS camera, 2048x2048 pixels, pixel size 6.5 $\mathrm{\mu m}$.

Illumination

• MPB Communications, 2RU-VFL-P-500-647-B1R, 647 nm laser, 500 mW.

• Thorlabs, DL5146-101S, 405 nm laser, 40 mW.

• Thorlabs, M660L3, 660 nm LED, 640 mW, for bright field illumination.

Other equipments

• National Instrument, USB-6008, DAQ card.

Filters

• Semrock, Di02-R635, dichroic mirror

• Semrock, FF01-708/75-25-D, band pass filter

Citation:

David J. Schodt, Farzin Farzam, Sheng Liu, and Keith A. Lidke, "Automated multi-target super-resolution microscopy with trust regions," Biomed. Opt. Express 14, 429-440 (2023)