Axial Scanning Lightsheet Microscope

Location: PAIS 2413

Equipment List

Microscope body

• Home built base.

• Sutter Instrument, MP-285, 3-Axis motorized stage, travel 25 mm, instrument resolution 40 nm.

Objective

• Special Optics, 54-10-12, multi-immersion, NA 0.4 @ RI 1.45, WD 12 mm @ all RI, FOV 1.2 mm.

Cameras

• Hamamatsu, C11440-22CU, sCMOS camera, 2048x2048 pixels, pixel size 6.5 $\mathrm{\mu m}$.

Illumination

• Coherent OBIS laser box.

  • LX 488-50C, 488 nm laser, 50 mW

  • LS 561-50, 561 nm laser, 50 mW

  • LX 637-140C, 637 nm laser, 140 mW

Other equipments

• Cambridge Technology, CRS 4 kHz, resonant mirror galvanometer

• National Instrument,PCIe-7852, Virtex-5 LX50 FPGA card.

• Equipment Solutions, LFA-2010, linear focus actuator, travel 10 mm, response time 3 ms @ 10 $\mathrm{\mu m}$ travel.

Filters

• Semrock, FF01-525/30-25, band pass filter

• Semrock, FF01-605/15-25, band pass filter

• Semrock, BLP01-647R-25, long pass filter

Citation:

Hassan Dibaji, Md Nasful Huda Prince, Yating Yi, Hu Zhao, and Tonmoy Chakraborty, "Axial scanning of dual focus to improve light sheet microscopy"

Md Nasful Huda Prince, Benjamin Garcia, Cory Henn, Yating Yi, Etsuo A. Susaki, Yuki Watakabe, Tomomi Nemoto, Keith A Lidke, Hu Zhao, Irene Salinas Remiro, Sheng Liu, Tonmoy Chakraborty, "Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging"