HSM

PAIS Multiscale Microscopy Center

HyperSpectral Microscope (HSM)

Location: PAIS 2409

Equipment List

Microscope body

  • Olympus IX71 Microscope Base.

  • MadCityLabs, Nano-LPS200, XYZ piezo stage, travel 200 μm\mathrm{\mu m}.

Objectives

  • Olympus, UPlanSApo 60X, Water Immersion, NA 1.2, WD 0.28 mm, FN 26.5.

  • Olympus, PlanApo N 60X, oil Immersion, NA 1.42, WD 0.15 mm, FN 26.5.

  • Olympus, UPlanFL N 10X, air Immersion, NA 0.3, WD 10 mm, FN 26.5.

Cameras

  • Andor, ZYLA-4.2P-USB3, sCMOS camera, 2048x2048 pixels, pixel size 6.5 μm\mathrm{\mu m}.

  • Andor, Luca R, EMCCD camera, 1004x1002 pixels, pixel size 8 μm\mathrm{\mu m}.

Illumination

  • Excelitas, X-Cite 120Q, Mercury lamp for widefield fluorescence illumination

  • Newport SpecraPhysics, PC13781, 488 nm laser, 40 mW.

Other equipments

  • Cambridge Technology, 6210H, galvo mirror.

  • National Instrument, PCIe-6343, DAQ card.

Filters

  • Widefield:

    • Semrock, LPD01-488RS-25x36x2, dichroic mirror

    • Semrock, LP02-488RU-25, long pass filter

  • Line scan: Semrock, LP02-488RU-25, long pass filter

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